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Blood Serum (/ˈsɪərəm/) is the fluid and solute component of blood which does not play a role in clotting.^[1] It may be defined as blood plasma without fibrinogens. Serum includes all proteins not used in blood clotting; all electrolytes, antibodies, antigens, hormones; and any exogenous substances (e.g., drugs or microorganisms). Serum does not contain white blood cells (leukocytes), red blood cells (erythrocytes), platelets, or clotting factors.

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The study of serum is serology. Serum is used in numerous diagnostic tests as well as blood typing. Measuring the concentration of various molecules can be useful for many applications, such as determining the therapeutic index of a drug candidate in a clinical trial.^[2]

To obtain serum, a blood sample is allowed to clot (coagulation). The sample is then centrifuged to remove the clot and blood cells, and the resulting liquid supernatant is serum.^[3]

Serum is an essential factor for the self-renewal of embryonic stem cells in combination with the cytokine leukemia inhibitory factor.^{[citation needed]}

Clinical and laboratory uses[edit]

The serum of convalescent patients successfully recovering (or already recovered) from an infectious disease can be used as a biopharmaceutical in the treatment of other people with that disease, because the antibodies generated by the successful recovery are potent fighters of the pathogen. Such convalescent serum (antiserum) is a form of immunotherapy.

Serum is also used in protein electrophoresis, due to the lack of fibrinogen which can cause false results.

Purification strategies[edit]

Blood serum and plasma are some of the largest sources of biomarkers, whether for diagnostics or therapeutics. Its vast dynamic range, further complicated by the presence of lipids, salts, and post-translational modifications, as well multiple mechanisms of degradation, presents challenges in analytical reproducibility, sensitivity, resolution, and potential efficacy. For analysis of biomarkers in blood serum samples, it is possible to do a pre-separation by free-flow electrophoresis that usually consists of a depletion of serum albumin protein.^[4] This method enables greater penetration of the proteome via separation of a wide variety of charged or chargeable analytes, ranging from small molecules to cells.

Usage note[edit]

Like many other mass nouns, the word serum can be pluralized when used in certain senses. To speak of multiple serum specimens from multiple people (each with a unique population of antibodies), physicians sometimes speak of sera (the Latin plural, as opposed to serums).

See also[edit]

References[edit]

• Martin, Elizabeth A., ed. (2007). Concise Medical Dictionary (7th ed.). Oxford, England: Oxford University Press. ISBN978-0-19-280697-0. Retrieved 8 September 2009.
• Wang, Wendy; Srivastava, Sudhir (2002). 'Serological Markers'. In Lester Breslow (ed.). Encyclopedia of Public Health. 4. New York, New York: Macmillan Reference USA. pp. 1088–1090.

1. ^'serum'. The Free Dictionary. Retrieved 2019-10-06.
2. ^Kaplan, Larry (2005-10-06). 'Serum Toxicology'(PDF). Clinical Pathology/Laboratory Medicine 2005. Columbia University. Retrieved 2020-01-28.
3. ^Thavasu PW, Longhurst S, Joel SP, Slevin ML, Balkwill FR (1992). 'Measuring cytokine levels in blood. Importance of anticoagulants, processing, and storage conditions'. J Immunol Methods. 153 (1–2): 115–24. doi:10.1016/0022-1759(92)90313-i. PMID1381403.CS1 maint: multiple names: authors list (link)
4. ^Nissum M., Foucher AL.: Analysis of human plasma proteins: a focus on sample collection and separation using free-flow electrophoresis. In: Expert Rev Proteomics. Nr. 5, 2008, S. 571–87.

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